Etching,Freeze

《英文msh词典》Etching,Freeze
[入口词] Etching,Freeze
[主题词] Freeze Etching
[英文释义] A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and,thus,allows for the sublimation of water during the freeze-drying process to etch the surfaces.